Composite

Part:BBa_K277135:Design

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)


3L.3_23.A2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3146
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 4476
    Illegal XbaI site found at 4503
    Illegal XbaI site found at 9454
    Illegal SpeI site found at 376
    Illegal SpeI site found at 2712
    Illegal SpeI site found at 6193
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3146
    Illegal NheI site found at 1950
    Illegal NheI site found at 2226
    Illegal NheI site found at 4372
    Illegal NheI site found at 6436
    Illegal SpeI site found at 376
    Illegal SpeI site found at 2712
    Illegal SpeI site found at 6193
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3146
    Illegal BglII site found at 6662
    Illegal BamHI site found at 8107
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3146
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 4476
    Illegal XbaI site found at 4503
    Illegal XbaI site found at 9454
    Illegal SpeI site found at 376
    Illegal SpeI site found at 2712
    Illegal SpeI site found at 6193
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3146
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 4476
    Illegal XbaI site found at 4503
    Illegal XbaI site found at 9454
    Illegal SpeI site found at 376
    Illegal SpeI site found at 2712
    Illegal SpeI site found at 6193
    Illegal AgeI site found at 3256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2922
    Illegal SapI site found at 3099


Design Notes

Constructed using USER assembly method described in RFC38


Source

Synthetic Yeast

References